Fig. 2

Germ cell meiotic progress of germ cells of Alligator sinensis collected from different dph groups. (A) Chromatin characteristics of germ cells at different meiotic stages, including leptotene, zygotene, pachytene, and diplotene.The left panel represented lepotene and zygotene oocytes observed within the Nest (white solid boxes), the right panel indicated the pachytene and diplotene oocytes presented advanced interaction with varying number of GCs (*) within the Pmf (white dotted boxes). (B) The proportion of germ cells within the mitotic and different meiosis progress of Alligator sinensis from 1-dph, 15-dph, 30-dph, 60-dph, 90-dph, and 300-dph respectively. (C) The mRNA expression of pre-meiotic marker STRA8, SCYP3 encoding synaptonemal complex protein, and DMC1 encoding a meiotic recombinase relative to the house-keeping gene RPL8. The significant differences are indicated with different letters (P < 0.05), the same letter means no significant difference (P > 0.05), the same as below