Table 2 Details of primers information used for RT-PCR
Genes | Accession number (Source) | Primer sequences (5′-3′) | Production size (bp) | Amplification efficiency(%) | |
|---|---|---|---|---|---|
Forward | Reverse | ||||
FSH | NM_001287605.1 (A. mississippiensis) | GTGAATGCCACTTGGTGCTC | TTGTTCCTCCCCCTTTACGC | 361 | 95.8% |
FSHR | XM_006014720.2 (A. sinensis) | GAGCATGGTCCTCCTATGCC | TTGTGGTGGCCTGTTCAAGT | 343 | 104.3% |
STRA8 | XM_025204830.1 (A. sinensis) | CACCAACGTTCTACCCCAGT | TGTCGTGCCTGAGATAAGCG | 357 | 101.6% |
SYCP3 | XM_019489452.1 (A. mississippiensis) | CGGAGGGGGAAGAGAAACAC | AGACTTCCGAACAGTTGCCA | 723 | 103.5% |
DMC1 | XM_006029850.2 (A. sinensis) | GTTTTTCCCAGGCCAAACTCA | GCTTCCTCGTGGAACTTTGC | 797 | 99.6% |
RPL8 | XM_006015645 (A. sinensis) | GGTGTGGCTATGAATCCTGT | ACGACGAGCAGCAATAAGAC | 237 | 96.6% |