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Fig. 2 | BMC Zoology

Fig. 2

From: Drosophila melanogaster chemical ecology revisited: 2-D distribution maps of sex pheromones on whole virgin and mated flies by mass spectrometry imaging

Fig. 2

The distribution of the female sex pheromones 7,11-HD and 7,11-ND within one set of legs originating from one six-day-old virgin D. melanogaster female. The dataset was obtained by MALDI-TOF MSI in positive ion mode with LiDHB matrix applied using SunChrom MALDI spotter. (a) A 2-D ion intensity map of 7,11-HD at m/z 383.42 ([M + Li]+) with signal intensity displayed in voxel view and rainbow-color coded within a mass window of ±1.0 Da. (b) The observed mass shift of m/z 383.4 represented as a 2-D ion intensity map in PlusMinus 1 color scale. (c) The mass shift correction of m/z 383.42 signal made after applying the recalibration method. (d) A 2-D ion intensity map of 7,11-ND at m/z 411.45 ([M + Li]+) with signal intensity displayed in voxel view and rainbow-color coded within a mass window of ±1.0 Da. (e) The observed mass shift of m/z 411.45 represented as a 2-D ion intensity map in PlusMinus 1 color scale. (f) The mass shift correction of m/z 411.45 signal made after applying the recalibration

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