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Fig. 3 | BMC Zoology

Fig. 3

From: Moonlight is a key entrainer of lunar clock in the brain of the tropical grouper with full moon preference

Fig. 3

Weekly changes in the transcript levels of crys in the diencephalon and pituitary gland of honeycomb grouper. Fish (6 to 13 individuals per each sampling point) were collected at the new moon (NM), first quarter moon (W01 and W11), full moon (W02 and W12) and last quarter moon (W03 and W13) and tissue was taken at ZT18. The mRNA abundance of crys was measured using qPCR. The data were normalized by determining the amount of ef1α. Each point shows the fold change in the expression levels from the new moon. Each data is shown as means ± SEM. Moon phases are shown on each figure. Different letters indicate statistically significant difference one-way ANOVA; cry1 (P < 0.05 from W01 to W03 and P < 0.01 from W11 to W13), cry2 (P < 0.01 from W01 to W03 and P < 0.01 from W11 to W13), and cry3 (P < 0.01 from W01 to W03 and P < 0.05 from W11 to W13) for the diencephalon; cry1 (P < 0.01 from W01 to W03 and P < 0.05 from W11 to W13), cry2 (P < 0.01 from W01 to W03 and P = 0.11 from W11 to W13), and cry3 (P < 0.05 from W01 to W03 and P = 0.25 from W11 to W13) for the pituitary gland. Asterisks show significant difference (P < 0.05) from the new moon. The source data and statistical analysis data are included in Additional files 3 and 4, respectively

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