Fig. 10

Neurosensory and excretory structures in Eudiplozoon nipponicum. A. General view of the forebody tegument, showing the distribution of sensory structures. White circles indicate a few examples of their innervation. CLSM, anti-α-tubulin-FITC. B. Detailed view of the tegument with labelled sensory structures. White rectangles indicate sensory cilia. CLSM, anti-α-tubulin-FITC/Hoechst. The inset in B shows two uniciliated receptors in another optical section, with shown phalloidin-TRITC counterstaining. Note the raised receptor with prominent innervation surrounded by muscles on the left of the image. C. High magnification showing uniciliated sensory structure with innervation. CLSM, anti-α-tubulin-FITC/Hoechst. D. A uniciliated sensory structure with visible dendrite. TEM. E-G. Putative innervation of sensory structures detected in the syncytium above the basal lamina (E) and between the circular (F) and longitudinal (G) muscle fibres. FE, TEM. The inset in E shows a putative nerve fibre in cross-ultrathin section. TEM. H. A longitudinal section of the innervation of a uniciliated sensory structure. TEM. I. Detailed view of the tegument, showing the sensory structures with innervation (white rectangle) and flame cells (arrowheads). CLSM, anti-α-tubulin-FITC/Hoechst. J. Detail of nerve fibres and a single flame cell. CLSM, anti-α-tubulin-FITC/Hoechst. black arrowheads – flame cells, cm – circular muscle fibres, de – dendrite, lm – longitudinal muscle fibres, mls – structures resembling nerve fibres with myelin sheaths, s – sensory structure, sc – syncytium, white arrowhead – centrally located innervation