Fig. 4From: Moonlight is a key entrainer of lunar clock in the brain of the tropical grouper with full moon preferenceEffect of nocturnal light irradiance on the transcript levels of crys in the diencephalon and pituitary gland of honeycomb grouper. After acclimating under a programmable condition (LD = 14:10), fish (3 to 5 individuals per each sampling point) were exposed to artificial full moon and new moon condition and tissues were collected in one-week interval (W1 to W4) at ZT18. The mRNA abundance of crys was measured using qPCR. The data were normalized by determining the amount of β-actin and ef1α. Each point shows comparison of the fold change in the expression levels between the artificial new moon and full moon. Each data is shown as means ± SEM. Different letters indicate statistically significant difference (one-way ANOVA); cry1 (P = 0.10), cry2 (P < 0.05), and cry3 (P < 0.05) for the diencephalon; cry1 (P < 0.05), cry2 (P < 0.05), and cry3 (P = 0.23) for the pituitary gland. Asterisks show statistical difference (P < 0.05) from the new moon condition. The source data and statistical analysis data are included in Additional files 3 and 4, respectivelyBack to article page