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Table 2 Mortality of female Panamanian golden frogs (Atelopus zeteki) after 2-weeks of natural breeding and in response to GnRHa treatments of 0.05, 0.1, 0.2, or 0.4 μg/g body weight. Data reflects the number and percent (%) of females that died after oviposition, and those that died without ovipositing

From: Influence of oviposition-inducing hormone on spawning and mortality in the endangered Panamanian golden frog (Atelopus zeteki)

Treatment

N

# Deaths (%) of Total N

Rate of Mortality (%)

Laid then died

Death if no eggs laid

No-hormone

111

20/111 (18.0)

12/102 (11.7)

8/9 (89.0)

All GnRHa

90

20/90 (22.2)

8/49 (16.3)

12/41 (29.3)

P-value

–

0.46

0.44

0.00008

GnRHa μg/g BW

N

# Deaths (%)

Laid then died

Death if no eggs laid

0.05

23

5/23 (21.7)

2/15 (9.1)

3/8 (37.5)

0.10

25

5/25 (20.0)

2/12 (10.0)

3/13 (23.1)

0.20

22

4/22 (18.2)

2/13 (18.2)

2/9 (22.2)

0.40

20

6/20 (30.0)

2/8 (25.0)

4/11 (36.4)

P-value

–

0.80

0.91

0.87