Fig. 7

Hindbody tegument organisation and innervation in Eudiplozoon nipponicum. A. General view of the hindbody bearing the haptor with clamps. Note the tegumentary annular ridges, lobular extensions and the row of non-ciliated papillae. SEM. B. A general view of the hindbody nervous system. CLSM, anti-α-tubulin-FITC/Hoechst. C. Detail of hindbody nerves, comprising the ventral cord and innervation to the haptor clamps. The micrograph shows the area marked by a white rectangle in B. CLSM, anti-α-tubulin-FITC. D. Hindbody stained with hydrochloric carmine, showing putative unicellular glands. CLSM, output image not coloured. E. Histological section showing the hindbody transverse tegumentary annular ridges and body wall musculature. LM, MT. F. Semithin section of tegument with ridges (black rectangle), revealing the organisation of individual muscle layers. LM, Toluidine blue. G. Ultrathin section showing circular and longitudinal muscles. TEM. H. Ultrastructural organisation of the haptor tegument. Note the gland cell with numerous secretory bodies and released secretory bodies within the syncytium. TEM. I. Fine structure of the hindbody tegument and body wall musculature. TEM. J. Detailed view showing the fine structure of the tegumentary ridge (similar to the area demarked by a black rectangle in F). TEM. apm – apical plasma membrane, asterisk – secretory bodies, black arrowheads – non-ciliated papillae, cl – clamp, cm – circular muscle fibres, dm – diagonal muscle fibres, g – gland cells, green arrowhead – interruption of basal lamina, h – haptor, ic – innervation of the haptor clamps, le – lobular extensions, lm – longitudinal muscle fibres, m – body wall musculature, mi – mitochondria, nf – nerve fibres innervating the clamp (nerve plexus), p – parenchyma, pm – perpendicular muscle fibres, rm – muscle fibres within tegumentary ridges, sc – syncytium, teg – tegument, tr – annular transverse ridges, vn – ventral nerve cord